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at180 antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher at180 antibody
    At180 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/at180+antibody/at180+antibody/pm40473739-179-0-15
    Average 90 stars, based on 1 article reviews
    at180 antibody - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr23166,71 was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Compositions and methods for suppressing MSUT2
    Article Snippet: Sections were treated for endogenous peroxidases with 3% hydrogen peroxide in PBS (pH 7.4), blocked in 5% non-fat milk in PBS, and incubated with one of the following primary antibodies overnight at 4° C.: MC1 (G. Carmel, et al., J Biol Chem 271, 32789-32795 (1996)), AT180 (ThermoScientific, catalog MN1040), GFAP (Dako Cytomation Z0334), Iba1 (Wako 019-19741), MSUT2 (C. R. Guthrie, et al., Hum Mol Genet 18, 1825-1838 (2009)), PABPN1 (Abcam ab81224), tau (Rb17025, (T. Ishihara, et al., American Journal of Pathology 158, 555-562 (2001)), or NeuN (Millipore MAB377).

    Article Title: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging.
    Article Snippet: PHF1 (1:50,000, gift from Peter Davies), AT8 (1:10,000, Thermo), AT180 (1:50,000, Thermo), AT270 (1:10,000, Thermo), total tau (1:75,000, A0024, Dako), GAPDH (1:20,000, Invitrogen).

    Article Title: APPsα Rescues Tau-Induced Synaptic Pathology
    Article Snippet: The following antibodies were used: AT8 (mouse monoclonal, 1:500, #MN1020, Thermo Fisher Scientific), AT180 (mouse monoclonal, 1:1000, #MN1040, Thermo Fisher Scientific).

    Article Title: TMEM106B coding variant is protective and deletion detrimental in a mouse model of tauopathy.
    Article Snippet: Primary antibodies included: CP27 (human tau 130–150, gift from Peter Davies), AT8 (pSer202, Thr205, ThermoFisher MN1020), PHF-1 (pSer396/404, gift from Peter Davies), AT100 (pThr212,Ser214, ThermoFisher MN1060), AT180 (pThr231, ThermoFisher MN1040), AT270 (pThr181, ThermoFisher MN1050); TAU-5 (total tau, Santa Cruz sc-58860), and Ck anti-GAPDH (Millipore AB2302).

    Article Title: Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration.
    Article Snippet: The following antibodies were used in this study: Tau5 (total tau; generously provided by Dr. Lester Binder), DAPI (Sigma), Alexa488 anti-rabbit (Life Technologies, Carlsbad, CA, USA), Alexa568 anti-mouse (Life Technologies, Carlsbad, CA, USA), beta III Tubulin (Promega), LAMP1 (Abcam, ab24170), AT180 (ptau-Thr231; Thermo Fisher Scientific, MN1040), EEA1 (Abcam, ab2900), Cathepsin D (generously provided by Dr. Stuart Kornfeld, Washington University in St Louis), Rab7 (Cell Signaling Technology, 9367S), TFEB (Abcam, ab270604), pTFEB (pSer122, Cell Signaling Technology, 86843S) and Ubiquitin (Cell Signaling Technology, 3933S).

    Article Title: Triggering receptor expressed on myeloid cells 2 deficiency exacerbates injury-induced inflammation in a mouse model of tauopathy
    Article Snippet: Sections were blocked in 5% NGS/0.3% Triton X-100 in 1× PBS for 1 h. The following primary antibodies were added overnight at 4°C: Iba1 (1:1,000; Wako Pure Chemical Industries), CD45 (1:500; AbD Serotec), CD68(1:500; Bio-Rad), F4/80 (1:500; AbD Serotec), doublecortin (1:250; Cell Signaling), APP (1:1000; Invitrogen), and AT180 (1:500; Thermo Fisher Scientific).

    Article Title: Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors.
    Article Snippet: Primary antibodies, AT180 or 4G8, and the secondary antibody Alexa Fluor 555, were purchased from Thermo Fisher and used without further purification.

    Avidin-Biotin Assay:

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr23166,71 was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Compositions and methods for suppressing MSUT2
    Article Snippet: Sections were treated for endogenous peroxidases with 3% hydrogen peroxide in PBS (pH 7.4), blocked in 5% non-fat milk in PBS, and incubated with one of the following primary antibodies overnight at 4° C.: MC1 (G. Carmel, et al., J Biol Chem 271, 32789-32795 (1996)), AT180 (ThermoScientific, catalog MN1040), GFAP (Dako Cytomation Z0334), Iba1 (Wako 019-19741), MSUT2 (C. R. Guthrie, et al., Hum Mol Genet 18, 1825-1838 (2009)), PABPN1 (Abcam ab81224), tau (Rb17025, (T. Ishihara, et al., American Journal of Pathology 158, 555-562 (2001)), or NeuN (Millipore MAB377).

    Article Title: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging.
    Article Snippet: PHF1 (1:50,000, gift from Peter Davies), AT8 (1:10,000, Thermo), AT180 (1:50,000, Thermo), AT270 (1:10,000, Thermo), total tau (1:75,000, A0024, Dako), GAPDH (1:20,000, Invitrogen).

    Article Title: APPsα Rescues Tau-Induced Synaptic Pathology
    Article Snippet: The following antibodies were used: AT8 (mouse monoclonal, 1:500, #MN1020, Thermo Fisher Scientific), AT180 (mouse monoclonal, 1:1000, #MN1040, Thermo Fisher Scientific).

    Article Title: TMEM106B coding variant is protective and deletion detrimental in a mouse model of tauopathy.
    Article Snippet: Primary antibodies included: CP27 (human tau 130–150, gift from Peter Davies), AT8 (pSer202, Thr205, ThermoFisher MN1020), PHF-1 (pSer396/404, gift from Peter Davies), AT100 (pThr212,Ser214, ThermoFisher MN1060), AT180 (pThr231, ThermoFisher MN1040), AT270 (pThr181, ThermoFisher MN1050); TAU-5 (total tau, Santa Cruz sc-58860), and Ck anti-GAPDH (Millipore AB2302).

    Article Title: Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration.
    Article Snippet: The following antibodies were used in this study: Tau5 (total tau; generously provided by Dr. Lester Binder), DAPI (Sigma), Alexa488 anti-rabbit (Life Technologies, Carlsbad, CA, USA), Alexa568 anti-mouse (Life Technologies, Carlsbad, CA, USA), beta III Tubulin (Promega), LAMP1 (Abcam, ab24170), AT180 (ptau-Thr231; Thermo Fisher Scientific, MN1040), EEA1 (Abcam, ab2900), Cathepsin D (generously provided by Dr. Stuart Kornfeld, Washington University in St Louis), Rab7 (Cell Signaling Technology, 9367S), TFEB (Abcam, ab270604), pTFEB (pSer122, Cell Signaling Technology, 86843S) and Ubiquitin (Cell Signaling Technology, 3933S).

    Article Title: Triggering receptor expressed on myeloid cells 2 deficiency exacerbates injury-induced inflammation in a mouse model of tauopathy
    Article Snippet: Sections were blocked in 5% NGS/0.3% Triton X-100 in 1× PBS for 1 h. The following primary antibodies were added overnight at 4°C: Iba1 (1:1,000; Wako Pure Chemical Industries), CD45 (1:500; AbD Serotec), CD68(1:500; Bio-Rad), F4/80 (1:500; AbD Serotec), doublecortin (1:250; Cell Signaling), APP (1:1000; Invitrogen), and AT180 (1:500; Thermo Fisher Scientific).

    Article Title: Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors.
    Article Snippet: Primary antibodies, AT180 or 4G8, and the secondary antibody Alexa Fluor 555, were purchased from Thermo Fisher and used without further purification.

    Incubation:

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr23166,71 was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Compositions and methods for suppressing MSUT2
    Article Snippet: Sections were treated for endogenous peroxidases with 3% hydrogen peroxide in PBS (pH 7.4), blocked in 5% non-fat milk in PBS, and incubated with one of the following primary antibodies overnight at 4° C.: MC1 (G. Carmel, et al., J Biol Chem 271, 32789-32795 (1996)), AT180 (ThermoScientific, catalog MN1040), GFAP (Dako Cytomation Z0334), Iba1 (Wako 019-19741), MSUT2 (C. R. Guthrie, et al., Hum Mol Genet 18, 1825-1838 (2009)), PABPN1 (Abcam ab81224), tau (Rb17025, (T. Ishihara, et al., American Journal of Pathology 158, 555-562 (2001)), or NeuN (Millipore MAB377).

    Article Title: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging.
    Article Snippet: PHF1 (1:50,000, gift from Peter Davies), AT8 (1:10,000, Thermo), AT180 (1:50,000, Thermo), AT270 (1:10,000, Thermo), total tau (1:75,000, A0024, Dako), GAPDH (1:20,000, Invitrogen).

    Article Title: APPsα Rescues Tau-Induced Synaptic Pathology
    Article Snippet: The following antibodies were used: AT8 (mouse monoclonal, 1:500, #MN1020, Thermo Fisher Scientific), AT180 (mouse monoclonal, 1:1000, #MN1040, Thermo Fisher Scientific).

    Article Title: TMEM106B coding variant is protective and deletion detrimental in a mouse model of tauopathy.
    Article Snippet: Primary antibodies included: CP27 (human tau 130–150, gift from Peter Davies), AT8 (pSer202, Thr205, ThermoFisher MN1020), PHF-1 (pSer396/404, gift from Peter Davies), AT100 (pThr212,Ser214, ThermoFisher MN1060), AT180 (pThr231, ThermoFisher MN1040), AT270 (pThr181, ThermoFisher MN1050); TAU-5 (total tau, Santa Cruz sc-58860), and Ck anti-GAPDH (Millipore AB2302).

    Article Title: Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration.
    Article Snippet: The following antibodies were used in this study: Tau5 (total tau; generously provided by Dr. Lester Binder), DAPI (Sigma), Alexa488 anti-rabbit (Life Technologies, Carlsbad, CA, USA), Alexa568 anti-mouse (Life Technologies, Carlsbad, CA, USA), beta III Tubulin (Promega), LAMP1 (Abcam, ab24170), AT180 (ptau-Thr231; Thermo Fisher Scientific, MN1040), EEA1 (Abcam, ab2900), Cathepsin D (generously provided by Dr. Stuart Kornfeld, Washington University in St Louis), Rab7 (Cell Signaling Technology, 9367S), TFEB (Abcam, ab270604), pTFEB (pSer122, Cell Signaling Technology, 86843S) and Ubiquitin (Cell Signaling Technology, 3933S).

    Article Title: Triggering receptor expressed on myeloid cells 2 deficiency exacerbates injury-induced inflammation in a mouse model of tauopathy
    Article Snippet: Sections were blocked in 5% NGS/0.3% Triton X-100 in 1× PBS for 1 h. The following primary antibodies were added overnight at 4°C: Iba1 (1:1,000; Wako Pure Chemical Industries), CD45 (1:500; AbD Serotec), CD68(1:500; Bio-Rad), F4/80 (1:500; AbD Serotec), doublecortin (1:250; Cell Signaling), APP (1:1000; Invitrogen), and AT180 (1:500; Thermo Fisher Scientific).

    Article Title: Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors.
    Article Snippet: Primary antibodies, AT180 or 4G8, and the secondary antibody Alexa Fluor 555, were purchased from Thermo Fisher and used without further purification.

    Immunofluorescence:

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr23166,71 was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Compositions and methods for suppressing MSUT2
    Article Snippet: Sections were treated for endogenous peroxidases with 3% hydrogen peroxide in PBS (pH 7.4), blocked in 5% non-fat milk in PBS, and incubated with one of the following primary antibodies overnight at 4° C.: MC1 (G. Carmel, et al., J Biol Chem 271, 32789-32795 (1996)), AT180 (ThermoScientific, catalog MN1040), GFAP (Dako Cytomation Z0334), Iba1 (Wako 019-19741), MSUT2 (C. R. Guthrie, et al., Hum Mol Genet 18, 1825-1838 (2009)), PABPN1 (Abcam ab81224), tau (Rb17025, (T. Ishihara, et al., American Journal of Pathology 158, 555-562 (2001)), or NeuN (Millipore MAB377).

    Article Title: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging.
    Article Snippet: PHF1 (1:50,000, gift from Peter Davies), AT8 (1:10,000, Thermo), AT180 (1:50,000, Thermo), AT270 (1:10,000, Thermo), total tau (1:75,000, A0024, Dako), GAPDH (1:20,000, Invitrogen).

    Article Title: APPsα Rescues Tau-Induced Synaptic Pathology
    Article Snippet: The following antibodies were used: AT8 (mouse monoclonal, 1:500, #MN1020, Thermo Fisher Scientific), AT180 (mouse monoclonal, 1:1000, #MN1040, Thermo Fisher Scientific).

    Article Title: TMEM106B coding variant is protective and deletion detrimental in a mouse model of tauopathy.
    Article Snippet: Primary antibodies included: CP27 (human tau 130–150, gift from Peter Davies), AT8 (pSer202, Thr205, ThermoFisher MN1020), PHF-1 (pSer396/404, gift from Peter Davies), AT100 (pThr212,Ser214, ThermoFisher MN1060), AT180 (pThr231, ThermoFisher MN1040), AT270 (pThr181, ThermoFisher MN1050); TAU-5 (total tau, Santa Cruz sc-58860), and Ck anti-GAPDH (Millipore AB2302).

    Article Title: Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration.
    Article Snippet: The following antibodies were used in this study: Tau5 (total tau; generously provided by Dr. Lester Binder), DAPI (Sigma), Alexa488 anti-rabbit (Life Technologies, Carlsbad, CA, USA), Alexa568 anti-mouse (Life Technologies, Carlsbad, CA, USA), beta III Tubulin (Promega), LAMP1 (Abcam, ab24170), AT180 (ptau-Thr231; Thermo Fisher Scientific, MN1040), EEA1 (Abcam, ab2900), Cathepsin D (generously provided by Dr. Stuart Kornfeld, Washington University in St Louis), Rab7 (Cell Signaling Technology, 9367S), TFEB (Abcam, ab270604), pTFEB (pSer122, Cell Signaling Technology, 86843S) and Ubiquitin (Cell Signaling Technology, 3933S).

    Article Title: Triggering receptor expressed on myeloid cells 2 deficiency exacerbates injury-induced inflammation in a mouse model of tauopathy
    Article Snippet: Sections were blocked in 5% NGS/0.3% Triton X-100 in 1× PBS for 1 h. The following primary antibodies were added overnight at 4°C: Iba1 (1:1,000; Wako Pure Chemical Industries), CD45 (1:500; AbD Serotec), CD68(1:500; Bio-Rad), F4/80 (1:500; AbD Serotec), doublecortin (1:250; Cell Signaling), APP (1:1000; Invitrogen), and AT180 (1:500; Thermo Fisher Scientific).

    Article Title: Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors.
    Article Snippet: Primary antibodies, AT180 or 4G8, and the secondary antibody Alexa Fluor 555, were purchased from Thermo Fisher and used without further purification.

    Staining:

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr23166,71 was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Compositions and methods for suppressing MSUT2
    Article Snippet: Sections were treated for endogenous peroxidases with 3% hydrogen peroxide in PBS (pH 7.4), blocked in 5% non-fat milk in PBS, and incubated with one of the following primary antibodies overnight at 4° C.: MC1 (G. Carmel, et al., J Biol Chem 271, 32789-32795 (1996)), AT180 (ThermoScientific, catalog MN1040), GFAP (Dako Cytomation Z0334), Iba1 (Wako 019-19741), MSUT2 (C. R. Guthrie, et al., Hum Mol Genet 18, 1825-1838 (2009)), PABPN1 (Abcam ab81224), tau (Rb17025, (T. Ishihara, et al., American Journal of Pathology 158, 555-562 (2001)), or NeuN (Millipore MAB377).

    Article Title: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging.
    Article Snippet: PHF1 (1:50,000, gift from Peter Davies), AT8 (1:10,000, Thermo), AT180 (1:50,000, Thermo), AT270 (1:10,000, Thermo), total tau (1:75,000, A0024, Dako), GAPDH (1:20,000, Invitrogen).

    Article Title: APPsα Rescues Tau-Induced Synaptic Pathology
    Article Snippet: The following antibodies were used: AT8 (mouse monoclonal, 1:500, #MN1020, Thermo Fisher Scientific), AT180 (mouse monoclonal, 1:1000, #MN1040, Thermo Fisher Scientific).

    Article Title: TMEM106B coding variant is protective and deletion detrimental in a mouse model of tauopathy.
    Article Snippet: Primary antibodies included: CP27 (human tau 130–150, gift from Peter Davies), AT8 (pSer202, Thr205, ThermoFisher MN1020), PHF-1 (pSer396/404, gift from Peter Davies), AT100 (pThr212,Ser214, ThermoFisher MN1060), AT180 (pThr231, ThermoFisher MN1040), AT270 (pThr181, ThermoFisher MN1050); TAU-5 (total tau, Santa Cruz sc-58860), and Ck anti-GAPDH (Millipore AB2302).

    Article Title: Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration.
    Article Snippet: The following antibodies were used in this study: Tau5 (total tau; generously provided by Dr. Lester Binder), DAPI (Sigma), Alexa488 anti-rabbit (Life Technologies, Carlsbad, CA, USA), Alexa568 anti-mouse (Life Technologies, Carlsbad, CA, USA), beta III Tubulin (Promega), LAMP1 (Abcam, ab24170), AT180 (ptau-Thr231; Thermo Fisher Scientific, MN1040), EEA1 (Abcam, ab2900), Cathepsin D (generously provided by Dr. Stuart Kornfeld, Washington University in St Louis), Rab7 (Cell Signaling Technology, 9367S), TFEB (Abcam, ab270604), pTFEB (pSer122, Cell Signaling Technology, 86843S) and Ubiquitin (Cell Signaling Technology, 3933S).

    Article Title: Triggering receptor expressed on myeloid cells 2 deficiency exacerbates injury-induced inflammation in a mouse model of tauopathy
    Article Snippet: Sections were blocked in 5% NGS/0.3% Triton X-100 in 1× PBS for 1 h. The following primary antibodies were added overnight at 4°C: Iba1 (1:1,000; Wako Pure Chemical Industries), CD45 (1:500; AbD Serotec), CD68(1:500; Bio-Rad), F4/80 (1:500; AbD Serotec), doublecortin (1:250; Cell Signaling), APP (1:1000; Invitrogen), and AT180 (1:500; Thermo Fisher Scientific).

    Article Title: Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors.
    Article Snippet: Primary antibodies, AT180 or 4G8, and the secondary antibody Alexa Fluor 555, were purchased from Thermo Fisher and used without further purification.

    Next-Generation Sequencing:

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr23166,71 was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Compositions and methods for suppressing MSUT2
    Article Snippet: Sections were treated for endogenous peroxidases with 3% hydrogen peroxide in PBS (pH 7.4), blocked in 5% non-fat milk in PBS, and incubated with one of the following primary antibodies overnight at 4° C.: MC1 (G. Carmel, et al., J Biol Chem 271, 32789-32795 (1996)), AT180 (ThermoScientific, catalog MN1040), GFAP (Dako Cytomation Z0334), Iba1 (Wako 019-19741), MSUT2 (C. R. Guthrie, et al., Hum Mol Genet 18, 1825-1838 (2009)), PABPN1 (Abcam ab81224), tau (Rb17025, (T. Ishihara, et al., American Journal of Pathology 158, 555-562 (2001)), or NeuN (Millipore MAB377).

    Article Title: Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging.
    Article Snippet: PHF1 (1:50,000, gift from Peter Davies), AT8 (1:10,000, Thermo), AT180 (1:50,000, Thermo), AT270 (1:10,000, Thermo), total tau (1:75,000, A0024, Dako), GAPDH (1:20,000, Invitrogen).

    Article Title: APPsα Rescues Tau-Induced Synaptic Pathology
    Article Snippet: The following antibodies were used: AT8 (mouse monoclonal, 1:500, #MN1020, Thermo Fisher Scientific), AT180 (mouse monoclonal, 1:1000, #MN1040, Thermo Fisher Scientific).

    Article Title: TMEM106B coding variant is protective and deletion detrimental in a mouse model of tauopathy.
    Article Snippet: Primary antibodies included: CP27 (human tau 130–150, gift from Peter Davies), AT8 (pSer202, Thr205, ThermoFisher MN1020), PHF-1 (pSer396/404, gift from Peter Davies), AT100 (pThr212,Ser214, ThermoFisher MN1060), AT180 (pThr231, ThermoFisher MN1040), AT270 (pThr181, ThermoFisher MN1050); TAU-5 (total tau, Santa Cruz sc-58860), and Ck anti-GAPDH (Millipore AB2302).

    Article Title: Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration.
    Article Snippet: The following antibodies were used in this study: Tau5 (total tau; generously provided by Dr. Lester Binder), DAPI (Sigma), Alexa488 anti-rabbit (Life Technologies, Carlsbad, CA, USA), Alexa568 anti-mouse (Life Technologies, Carlsbad, CA, USA), beta III Tubulin (Promega), LAMP1 (Abcam, ab24170), AT180 (ptau-Thr231; Thermo Fisher Scientific, MN1040), EEA1 (Abcam, ab2900), Cathepsin D (generously provided by Dr. Stuart Kornfeld, Washington University in St Louis), Rab7 (Cell Signaling Technology, 9367S), TFEB (Abcam, ab270604), pTFEB (pSer122, Cell Signaling Technology, 86843S) and Ubiquitin (Cell Signaling Technology, 3933S).

    Article Title: Triggering receptor expressed on myeloid cells 2 deficiency exacerbates injury-induced inflammation in a mouse model of tauopathy
    Article Snippet: Sections were blocked in 5% NGS/0.3% Triton X-100 in 1× PBS for 1 h. The following primary antibodies were added overnight at 4°C: Iba1 (1:1,000; Wako Pure Chemical Industries), CD45 (1:500; AbD Serotec), CD68(1:500; Bio-Rad), F4/80 (1:500; AbD Serotec), doublecortin (1:250; Cell Signaling), APP (1:1000; Invitrogen), and AT180 (1:500; Thermo Fisher Scientific).

    Article Title: Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors.
    Article Snippet: Primary antibodies, AT180 or 4G8, and the secondary antibody Alexa Fluor 555, were purchased from Thermo Fisher and used without further purification.



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    a Western blot of soluble hippocampal lysates from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice were evaluated for markers of phosphorylated (AT8, <t>AT180,</t> PHF1) and total tau (Tau5). All samples were run on the same gel. b Compared to Qβ Control vaccinated mice, Qβ-PHF1 vaccinated mice showed a significant reduction in pathological AT180 + tau without any reduction in total physiologic tau. c . Immunohistochemistry of brain sections from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice staining for AT180 tau pathology in the cerebral cortex (CTX) and CA1 hippocampus. d There was a significant reduction in AT180 + tau pathology in both the cortex and hippocampus of Qβ-PHF1 vaccinated mice compared to Qβ Control mice ( e , f ). Western blot of soluble hippocampal lysates from Qβ Control and Qβ-AT8 vaccinated rTg4510 mice showed no significant reductions in pathological tau. All graphs display mean ± SEM. Student’s t -test ( b , d , f ). p < 0.05*; p < 0.001***; p < 0.0001****. Qβ Control ( n = 5), Qβ-AT8 ( n = 7), Qβ-PHF1 ( n = 8).
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    90
    Thermo Fisher pt231 antibody at180
    a Western blot of soluble hippocampal lysates from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice were evaluated for markers of phosphorylated (AT8, <t>AT180,</t> PHF1) and total tau (Tau5). All samples were run on the same gel. b Compared to Qβ Control vaccinated mice, Qβ-PHF1 vaccinated mice showed a significant reduction in pathological AT180 + tau without any reduction in total physiologic tau. c . Immunohistochemistry of brain sections from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice staining for AT180 tau pathology in the cerebral cortex (CTX) and CA1 hippocampus. d There was a significant reduction in AT180 + tau pathology in both the cortex and hippocampus of Qβ-PHF1 vaccinated mice compared to Qβ Control mice ( e , f ). Western blot of soluble hippocampal lysates from Qβ Control and Qβ-AT8 vaccinated rTg4510 mice showed no significant reductions in pathological tau. All graphs display mean ± SEM. Student’s t -test ( b , d , f ). p < 0.05*; p < 0.001***; p < 0.0001****. Qβ Control ( n = 5), Qβ-AT8 ( n = 7), Qβ-PHF1 ( n = 8).
    Pt231 Antibody At180, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/at180+antibody/pt231+antibody/pm40317322-50-15-18
    Average 90 stars, based on 1 article reviews
    pt231 antibody at180 - by Bioz Stars, 2026-10
    90/100 stars
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    90
    Thermo Fisher at180, mo mab antibody
    a Western blot of soluble hippocampal lysates from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice were evaluated for markers of phosphorylated (AT8, <t>AT180,</t> PHF1) and total tau (Tau5). All samples were run on the same gel. b Compared to Qβ Control vaccinated mice, Qβ-PHF1 vaccinated mice showed a significant reduction in pathological AT180 + tau without any reduction in total physiologic tau. c . Immunohistochemistry of brain sections from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice staining for AT180 tau pathology in the cerebral cortex (CTX) and CA1 hippocampus. d There was a significant reduction in AT180 + tau pathology in both the cortex and hippocampus of Qβ-PHF1 vaccinated mice compared to Qβ Control mice ( e , f ). Western blot of soluble hippocampal lysates from Qβ Control and Qβ-AT8 vaccinated rTg4510 mice showed no significant reductions in pathological tau. All graphs display mean ± SEM. Student’s t -test ( b , d , f ). p < 0.05*; p < 0.001***; p < 0.0001****. Qβ Control ( n = 5), Qβ-AT8 ( n = 7), Qβ-PHF1 ( n = 8).
    At180, Mo Mab Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/at180+antibody/at180+antibody/bio_rxiv__2025__04__09__648063-178-49-52
    Average 90 stars, based on 1 article reviews
    at180, mo mab antibody - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    Immunohistochemical labelling of Alzheimer’s disease neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. (a) Staining of AD and control frontal cortex tissue using monoclonal antibodies 7F2, AT180, 1F10, and AT100 is shown. Thin arrows indicate NFTs, arrow heads demonstrate neuritic plaques, thick arrows highlight neuropil threads. Scale bar of low magnification images is 100 μm, inset is 15 μm. (b) Semi-quantitative analysis of AD tau pathology in the frontal cortex is described in the “Materials and methods”. Data is presented as mean +/- SEM.

    Journal: Scientific Reports

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies

    doi: 10.1038/s41598-025-04291-y

    Figure Lengend Snippet: Immunohistochemical labelling of Alzheimer’s disease neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. (a) Staining of AD and control frontal cortex tissue using monoclonal antibodies 7F2, AT180, 1F10, and AT100 is shown. Thin arrows indicate NFTs, arrow heads demonstrate neuritic plaques, thick arrows highlight neuropil threads. Scale bar of low magnification images is 100 μm, inset is 15 μm. (b) Semi-quantitative analysis of AD tau pathology in the frontal cortex is described in the “Materials and methods”. Data is presented as mean +/- SEM.

    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr231 , was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Immunohistochemical staining, Staining, Control, Bioprocessing

    Immunohistochemical labelling of PSP and CBD neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. (a) Staining of PSP and CBD striatal tissue using monoclonal antibodies 7F2, AT180, and 1F10 is shown. Scale bar is 80 μm. (b) Semi-quantitative analysis of PSP and CBD tau pathology in the caudate and putamen is described in the “Materials and methods”. Data is presented as mean +/- SEM.

    Journal: Scientific Reports

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies

    doi: 10.1038/s41598-025-04291-y

    Figure Lengend Snippet: Immunohistochemical labelling of PSP and CBD neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. (a) Staining of PSP and CBD striatal tissue using monoclonal antibodies 7F2, AT180, and 1F10 is shown. Scale bar is 80 μm. (b) Semi-quantitative analysis of PSP and CBD tau pathology in the caudate and putamen is described in the “Materials and methods”. Data is presented as mean +/- SEM.

    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr231 , was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Immunohistochemical staining, Staining, Bioprocessing

    Immunohistochemical labelling of PiD neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. Staining of PiD hippocampal tissue using antibodies 7F2, AT180, and 1F10 is shown. Scale bar of low magnification images is 100 μm, inset is 15 μm.

    Journal: Scientific Reports

    Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies

    doi: 10.1038/s41598-025-04291-y

    Figure Lengend Snippet: Immunohistochemical labelling of PiD neuropathology. Immunohistochemical staining was performed as described in the “Materials and methods”. Staining of PiD hippocampal tissue using antibodies 7F2, AT180, and 1F10 is shown. Scale bar of low magnification images is 100 μm, inset is 15 μm.

    Article Snippet: AT180 is a mouse monoclonal antibody specific for tau phosphorylated at Thr231 , was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Immunohistochemical staining, Staining

    a Western blot of soluble hippocampal lysates from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice were evaluated for markers of phosphorylated (AT8, AT180, PHF1) and total tau (Tau5). All samples were run on the same gel. b Compared to Qβ Control vaccinated mice, Qβ-PHF1 vaccinated mice showed a significant reduction in pathological AT180 + tau without any reduction in total physiologic tau. c . Immunohistochemistry of brain sections from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice staining for AT180 tau pathology in the cerebral cortex (CTX) and CA1 hippocampus. d There was a significant reduction in AT180 + tau pathology in both the cortex and hippocampus of Qβ-PHF1 vaccinated mice compared to Qβ Control mice ( e , f ). Western blot of soluble hippocampal lysates from Qβ Control and Qβ-AT8 vaccinated rTg4510 mice showed no significant reductions in pathological tau. All graphs display mean ± SEM. Student’s t -test ( b , d , f ). p < 0.05*; p < 0.001***; p < 0.0001****. Qβ Control ( n = 5), Qβ-AT8 ( n = 7), Qβ-PHF1 ( n = 8).

    Journal: NPJ Vaccines

    Article Title: pS396/pS404 (PHF1) tau vaccine outperforms pS199/pS202 (AT8) in rTg4510 tauopathy model

    doi: 10.1038/s41541-025-01147-4

    Figure Lengend Snippet: a Western blot of soluble hippocampal lysates from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice were evaluated for markers of phosphorylated (AT8, AT180, PHF1) and total tau (Tau5). All samples were run on the same gel. b Compared to Qβ Control vaccinated mice, Qβ-PHF1 vaccinated mice showed a significant reduction in pathological AT180 + tau without any reduction in total physiologic tau. c . Immunohistochemistry of brain sections from Qβ Control and Qβ-PHF1 vaccinated rTg4510 mice staining for AT180 tau pathology in the cerebral cortex (CTX) and CA1 hippocampus. d There was a significant reduction in AT180 + tau pathology in both the cortex and hippocampus of Qβ-PHF1 vaccinated mice compared to Qβ Control mice ( e , f ). Western blot of soluble hippocampal lysates from Qβ Control and Qβ-AT8 vaccinated rTg4510 mice showed no significant reductions in pathological tau. All graphs display mean ± SEM. Student’s t -test ( b , d , f ). p < 0.05*; p < 0.001***; p < 0.0001****. Qβ Control ( n = 5), Qβ-AT8 ( n = 7), Qβ-PHF1 ( n = 8).

    Article Snippet: Antibody dilutions were as follows: AT180 (Thermo Fisher Scientific, MN1040) at 1:500, AT8 (Thermo Fisher Scientific, MN1020) at 1:500, Iba1 (WAKO, 019-19741) at 1:500, and CD45 (BioRad, MCA43R) at 1:250 incubated overnight at 4 °C.

    Techniques: Western Blot, Control, Immunohistochemistry, Staining